Shared Instrumentation Facility

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The Chemistry Department Shared Instrumentation Facility is housed in Room 357 of the Brown Building and is staffed by Dr. Chin Lin, Dr. Chunhua Hu, and Mr. Ronald McLurkin. The facility houses many of the Department's major research instruments including NMR spectrometers, mass spectrometers, polarimeter, electronic and vibrational spectrophotometers, X-ray diffractometers, and electron microscope.  Also housed here is a phosphorimager and a poster printer.  A diverse community of users access the facility, including faculty, graduate students, undergraduates, and numerous community members.  Colleagues access the facility from other departments, the NYU School of Medicine, and other local institutions.  

The instruments are open to all Chemistry Department research groups.  New users should contact Dr. Chin Lin for training schedules. Researchers outside of the Chemistry Department may also apply to Dr. Lin to use the instruments in the facility.

> Instrument Rate List
> Instrument Reservation System



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The Shared Instrumentation Facility was constructed through the support of the National Center for Research Resources, National Institutes of Health under Research Facilities Improvement Award Number C06 RR-16572-01.



Description of Instruments


Bruker AV-400 High Performance Digital NMR Spectrometer (400 MHz):


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The Bruker Avance-400 NMR spectrometer is equipped with two RF channels, a multinuclear broadband observe (BBO) probe covering the range from 15N to 31P nuclei, an inverse 1H (inner)/ 13C (outer) carbon (SEI) probe, and a GRASP II Gradient Spectroscopy accessory. The BBO probe is further equipped with a built-in Automatic Tuning and Matching Accessory (ATMA) for computer controlled X observation and 1 H decoupling tuning and matching. In addition, the BBO probe was prepared so that the outer coil can be tuned to 19F. A low temperature accessory, including 25 liter nitrogen dewar, nitrogen evaporator, and insulated transfer line, for operation down to -100 °C was also installed. This instrument is accessible to all chemistry research groups except every Thursday morning between 9:00 and 10:30 am (for liquid Nitrogen/Helium filling).

The Bruker Avance-400 NMR Spectrometer was acquired through the support of the National Science Foundation under Award Number CHE-01162222.


Bruker AV-500 High Performance Digital NMR Spectrometer (500 MHz):

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The Bruker Avance-500 NMR spectrometer is equipped with three RF channels, an inverse Triple Resonance Probe (TXI) for observation of 1H while decoupling 13C and 15N nuclei, an inverse (proton-detected) Broadband Probe (BBI) with broadband decoupling coil tunable from 109Ag to 31P nuclei, and a GRASP III three axis Gradient Spectroscopy accessory. The Bruker Avance-500 NMR spectrometer also equipped with a pre-cooling and stabilization accessory (BCU-05) for ultra-precise low temperature control from near ambient conditions down to -5 °C sample temperature ideal for DNA/RNA and peptide molecules.

The Bruker Avance-500 NMR Spectrometer was acquired through the support of the National Science Foundation under Award Number CHE-01162222.


Bruker AVIII-600 High Performance Digital NMR Spectrometer (600 MHz):

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The Bruker AVANCE III-600 NMR spectrometer is equipped with three RF channels, 5 mm sample diameter Inverse Quadruple Resonance Probe (QXI) for observation of 1H while decoupling 13C, 15N, and 31P nuclei, an multinuclear Broadband Fluorine Observe SmartPROBE (BBFO) with digital tuning covering the range from 15N to 31P and 19F nuclei with 1H decoupling, and a GRASP II Z-axis Gradient Spectroscopy accessory. Both QXI and BBFO probes equipped with a computer controlled Automatic Tuning and Matching (ATM) accessory for optimum automation. The Bruker AVANCE III-600 NMR spectrometer also equipped with a pre-cooling and stabilization accessory (BCU1) for ultra-precise low temperature control from near ambient conditions down to 0 °C sample temperature ideal for biopolymers. A triple resonance (1H, 13C, and 15N) CPTCI-cryoprobehead was added in April of 2015.
The Bruker AVANCE III-600 NMR Spectrometer was acquired through the support of New York University.

The CPTCI-cryoprobehead was acquired through the support of the National Institute of Health S10 grant under Award Number OD016343.


Agilent 1100 Series Capillary LCMSD Trap XCT Spectrometer:

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 The Agilent 1100 Series Capillary LCMSD Trap XCT system is a multi-pole ion-trap mass spectrometer designed to deliver state-of-the-art performance for API ( Atmospheric Pressure Ionization)-LCMS applications for examining polar organic and particularly biological compounds such as DNAs/RNAs and peptides directly in aqueous solution . This system is optimized for capillary LC but still offers a wide range of flow rates. This LCMSD is equipped with a Micro Vacuum Degasser, a Micro Auto-sampler, a Diode Array and Multiple Wavelength Detector, a Thermostatted Column Compartment, and an Ion-trap Mass Selective Detector with a maximum range of 4,000 amu. In addition to ESI ionization source, this system is also equipped with APCI ( Atomic Pressure Chemical Ionization) and APPI ( Atmospheric Pressure Photo Ionization) sources for wide range of samples. In generals, the total amounts of sample injected into the system are in the pmol/μL range with a flow rate from 5 to 200 μL per minute.

The Agilent LCMSD Trap XCT Mass Spectrometer was acquired through the support of the National Science Foundation under Award Number CHE-0234863.


Agilent 6224 Accurate-Mass TOF/LC/MS Spectrometer:

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The Agilent 6224 Accurate-Mass TOF LC/MS system is a High-Definition mass spectrometer capable of delivering exceptional sensitivity and excellent sub 1-ppm mass accuracy. This LC-TOF system is equipped with a vacuum degasser, a binary pump, a well-plate auto-sampler, a diode array and multiple wavelength detector (DAD), and a Time Of Flight (TOF) mass selective detector with a wide mass range of 20,000 m/z. In addition to the duo-ESI ionization source, this system is also equipped with an APCI (Atmospheric Pressure Chemical Ionization) source for wide range of samples with less polarity.

The Agilent 6224 TOF LC/MS Mass Spectrometer was acquired through the support of New York University.


Agilent 1100 Series LCMSD VL MS Spectrometer:

The Agilent 1100 Series LCMSD VL system is a single quadrupole mass spectrometer designed to deliver state-of-the-art performance for API (Atmospheric Pressure Ionization)-LCMS applications. This LCMSD is equipped with a vacuum degasser, a quadruple pump, a well-plate auto-sampler, a diode array and multiple wavelength detector (DAD), and a single quadrupole mass selective detector with a maximum mass range of 1,500 atomic mass unit. In addition to ESI ionization source, this system is also capable of running APCI (Atmospheric Pressure Chemical Ionization) source for wide range of samples with less polarity. In generals, the total concentration of sample injected into the system is in the pmol/µl range with a flow rate up to 1000 µL per minute. 

The Agilent 1100 Series LCMSD VL MS Spectrometer was acquired through the support of New York University.


Bruker UltrafleXtreme MALDI-TOF/TOF:

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The UltrafleXtreme is a MALDI (Matrix Assisted Laser Desorption/Ionization) tandem Mass Spectrometer especially designed for automated MS and MS/MS high throughput identification of proteins and peptides.  Tandem Mass Spectrometry is a technique that utilizes more than one mass selective stage in a mass spectrometer.  The most common form in practice is a two-stage arrangement to record MS/MS or (MS)2 spectra.  Bruker's patented LIFTTM technology allows acquiring full fragment ion spectra within one single scan and replaces traditional measurements of segmented spectra with stepwise-reduced reflector potentials.  The TOF/TOF technology provides enhanced ion optics for cutting-edge MS/MS experiments and second generation PANTM 'panoramic' resolution allows impressive simultaneous mass resolution across a wide mass range leading to reliable top-down sequencing capabilities.

The Bruker Maldi-TOF TOF UltrafleXtreme MS Spectrometer was acquired through the support of the National Science Foundation under Award Number CHE-0958457.


ThermoScientific Nicolet-6700 Fourier Transform Infrared Spectrometer (FTIR):


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The ThermoScientific Nicolet-6700 Fourier Transform Infrared Spectroscopy (FTIR) system lets you collect spectra in the mid-IR, far-IR, and near-IR spectra ranges, from 7,800 to 350 cm-1 with the resolution of 0.125 cm-1. This system is using the Gold mid-IR optics with a KBr beamsplitter, DLaTGS detector w/KBr window and liquid Nitrogen-cooled MCT-A detectors. In addition, a Smart iTR with single reflection Diamond plate HATR for liquid and powder samples is included. The system integrates advanced hardware features with the power and flexibility of OMNIC software.

The Thermo Nicolet-6700 FTIR Spectrometer was acquired through the support of New York University.


Cary 100 Bio UV-Visible Spectrophometer:



The Cary 100 Bio spectrophotometer is a mid-priced instrument, ideal for routine laboratory work and is controlled by the Cary WinUV software. This Windows based software features a modular design which makes it easy to use. It has a working range past 3.5 absorbance units (AU) and variable slits. The instrument comes with a 6 x 6 peltier multicell holder and a temperature controller unit. The peltier thermostattable multicell holder features, use of rotating magnetic fields to provide magnetic stirring in each of the 12 cells, uilt-in cell lifting, Peltier blocks fitted down both sides of the cuvette positions, and a water channel that evenly encircles the cell block. The temperature range is from about -10 deg C to 100 deg C, ideal for DNA/RNA melting temperature determination.

The Cary 100 Bio UV-Visible Spectrophometer was acquired through the support of New York University.


Storm PhosphorImager:

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The Molecular PhosphorImager systems Storm 840 provides users PhosphorImager capability for autoradiography, direct fluorescence for nucleic acid and protein gel analysis, and chemifluorescence for fast blot analysis without exposures. Please clean up the scanner screen with mixture of ethanol/water after each use.

The Storm PhosphorImager was acquired through the support of New York University.


Circular Dichroism Spectropolarimeter:

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The Department has an Aviv Stopped Flow CD Spectropolarimeter Model 202SF equipped with a thermostated five compartments cell holder. Circular Dichroism is an excellent tool for analyzing peptide/protein and nucleic acid (DNA and RNA) secondary structure in solution. It can be used to follow the changes in folding as a function of temperature, and is also useful for measuring protein-ligand and nucleic acid-ligand interactions.

The Circular Dichroism Spectropolarimeter was acquired through the support of New York University.


Perkin Elmer 2400 Series II CHNS/O Elemental Analyzer:

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The system can determine weight percent carbon, hydrogen, nitrogen, sulfur or oxygen in a variety of sample types, and operate in 3 modes: CHN, CHNS and Oxygen. Currently the system is only operating under the CHN mode. A typical measurement requires 1.5-2.5 mg of sample. Please contact Dr. Chunhua "Tony" Hu for training.


Bruker AXS SMART APEXII Single Crystal Diffractometer:

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It is equipped with an APEXII CCD area detector and an Oxford Cryosystems 700 plus Cooler. It is not only used for single crystal structure determination, also can be applied to powder sample for phase identification. Please visit the X-ray Diffraction Facility web page for more information.

The Bruker AXS SMART APEXII Single Crystal Diffractometer was acquired through the support of the Molecular Design Institute in the Department of Chemistry at New York University.


Bruker AXS D8 DISCOVER with GADDS Diffractometer:

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Please visit the X-ray Diffraction Facility web page for more information.

The X-ray microdiffractometer with GADDS was acquired through the support of the National Science Foundation under Award Numbers CRIF/CHE-0840277 and Award Number MRSEC/DMR-0820341.


Merlin FESEM:

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MERLIN (Carl Zeiss) field emission scanning electron microscope (FESEM): The Merlin FESEM features standard detectors, ‘SE2’ (Everhart-Thornley type) and ‘in lens’ (annular secondary electron).

The Merlin FESEM was acquired through the support of the MRI program of the National Science Foundation under Award Number DMR-0923251.

Please visit the Electron Microscopy Facility web page for more information.


EMS 150R ES Sputter Coater:


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The Electron Microscopy Sciences 150R ES Sputter Coater is a compact turbomolecular-pumped coating system suitable for SEM, TEM and many thin-film applications. This combined system is capable of performing both metal sputtering (usually gold) and carbon coating in one single compartment. The deposition inserts can be swapped in seconds and the intelligent system logic automatically recognizes which insert is in place and displays the appropriate operating settings. For more information please go to the following website link, http://www.emsdiasum.com/microscopy/products/equipment/turbo_evaporator.aspx


Cressington High Resolution Sputter Coater 208HR:


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The Cressington High Resolution Sputter Coater 208HR is designed for Field Emission‐SEM (FE-SEM) imaging where extremely thin, grain‐free, uniform coatings are required to eliminate charging and to improve contrast on low density materials. The target material is iridium to accomplish the above requirements. The HIGH and LOW chamber configuration allows easy adjustment of the working distance from target to sample. The MTM‐20 thickness controller has a resolution of better than 0.1 nm, enabling precise and reproducible thin coatings, especially in the range of 0.5 – 3 nm.


Horiba PTI QuantaMaster 400 Fluorescent Spectrometer:


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The Horiba PTI QuantaMaster 400 Fluorescent Spectrometer is an extremely sensitive bench-top fluorimeter with a signal to noise ratio of 20,000 to 1. With this high sensitivity it allows the minute traces of fluorescent materials to be detected and identified in samples. This system features state-of-the-art high throughput monochromators with a triple motorized grating turret for easy extension of the spectral range and motorized flipping mirrors to make it easy to work with additional light sources and detectors. The system is controlled with PTI’s FelixGX program for data acquisition and processing. The emission range for this system is from 185 nm to 680 nm with the detection limit of 400 attomolar of fluorescein solution in 0.1 M NaOH. It also equipped with an automated temperature controlling unit allowing precise variable temperature control from -25 °C to 105 °C.


Jasco Polarimeter P-2000:


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The Jasco Polarimeter P-2000 is designed as a customizable polarimeter with various options for an array of applications. The system can also be field upgraded as application requirements change. Options for wavelength filters and source lamps provide a wide range of analytical wavelengths from UV-Vis to NIR. The P-2000 offers a response speed as fast as six degrees per second to provide reproducible data for each measurement with a resolution of 0.0001°. A wide dynamic range of up to ±90° enables the system to measure chiral compounds over a broad range of concentrations. The sample chamber is equipped with an integrated temperature, the current temperature constantly updated on the instrument control screen.


Molecular Devices FlexStation 3 Microplate Reader:


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The Molecular Devices FlexStation 3 multi-mode microplate reader has a dual optical systems operating above and below the microplates, measuring absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence (5 detection modes). This compact benchtop system integrates multi-mode microplate optics with precision, programmable liquid handling for perfectly timed, automated biochemical and cell-based assays. Using an 8- or 16-channel pipettor, the FlexStation 3 reader offers added assay flexibility over dispenser-based systems by transferring reagents from 96 or 384 distinct wells in a source plate to a read plate simultaneously. In addition, users can define individual reagents and concentrations to be delivered to each well.


Malvern Zetasizer Nano Series:


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The Malvern Zetasizer nano series is the combinations of a particle size analyzer, zeta potential analyzer, molecular weight analyzer, protein mobility, and microrheology measurements. Particles and molecules from less than a nanometer in size to several microns can be analyzed by this instrument. This system is able to measure the following (1) size and microrheology using dynamic light scattering, (2) zeta potential and electrophoretic mobility using electrophoretic light scattering, and (3) molecular weight using static light scattering. In addition the system can be used in a flow configuration to connect to a GPC/SEC system for use as a chromatography size detector. For more detail information for this instrument please go to the following link, http://www.malvern.com/en/products/product-range/zetasizer-range/zetasizer-nano-range/default.aspx


Leica Confocal Microscope:


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The Leica TCS SP8 X Laser Confocal Microscope set-up includes two super-sensitive GaAsP hybrid detectors (with quantum efficiency twice as much as a standard PMT) and the state-of-the-art White Light Laser (WLL) source that can virtually output any wavelength from 470 to 670. Up to eight lines can be used simultaneously. The WLL source that can perfectly match the wavelength of virtually any fluorophore. By tuning both excitation and detection, complete excitation emission spectra can be acquired. With this spectral information, any dye can be optimally excited with minimum cross-excitation and specimen damage. As a consequence the choice of fluorescent labels is not limited any longer by the hardware. Another unique benefit of equipping the Leica TCS SP8 X with a WLL source is the ability of using an adjustable time gate to switch off the data collection during the white light laser pulse. This clever, filter-free method to remove unwanted signal from the image data produces the highest image contrast without any reflection. The Microscope set-up also includes a digital color camera Leica DFC310 FX, which provides images of fluorescence-labeled specimens with high color fidelity. Active cooling of sensor elements using a Peltier element creates noise-free images even at the lowest light intensities. Various acquisition modes such as binning allow for the precise monitoring of fast dynamic processes. The extremely high scanning frequency allows efficient documentation of 3D moving objects at full camera resolution. The camera delivers up to 71 images per second in 4 x 4 binning mode. The top scan rate allows fast focusing using the monitor and perfect parameter adjustment. At the same time, specimen photobleaching is avoided. Recording also can be performed in high-resolution mode. This instrument was purchased with a combination of MRSEC and departmental funds (prof. Stefano Sacanna startup).


TA Instrument Nano Isothermal Titration Calorimeter (Nano ITC LV):


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The TA Instrument Nano Isothermal Titration Calorimeter Low Volume (Nano ITC LV) consists of a measuring unit (calorimeter block and two non-removable reaction vessels), a 50 uL removable buret assembly with the stirring system, and a cleaning accessory. This system provides the maximum sensitivity and flexibility for the study of bimolecular binding. In addition, the Nano ITC low volume offers the lowest cell volume at 190 uL, to minimize sample consumption and at the same time provides sensitivity levels over two times better than previously achievable. For more information please click on the following link, http://www.tainstruments.com/pdf/brochure/Nano-ITC.pdf


Thermo Scientific DXR Raman Microscope:


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The Thermo Scientific DXR Raman microscope provides point and shoot Raman microscopy with exceptional sensitivity and spatial resolution. The microscope is equipped with two excitation lasers of wavelengths 780 nm and 532 nm, a full range grating (50-3500 cm-1 for 532 nm, 50-3300 cm-1 for 780 nm) and a high resolution grating(50-1800 cm-1 for both). The spectral resolution is 5.0 cm-1 for the full range and 2.0 cm-1 for the high resolution. The microscope is equipped with four long working distance Olympus objectives, 10X, 20X, 50Xand 100X and Brightfield/Darkfield illuminator. It also has a macro sampling adapter accessory that allows measurement on samples dissolved in solution. OMNIC Atlμs software provides hyperspectral mapping capability and image analysis.



The Horiba Smart SE Ellipsometer:


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The Smart SE Ellipsometer from Horiba providesfast and accurate thin film measurement. It characterizes thin film thickness(from a few nanometers to 20 μm), optical constants (n and k), and thin filmstructural properties (such as roughness and anisotropic layers etc.) Thespectral range of the instrument is 450-1000 nm and the resolution is > 3nm.The angle of incidence is from 45°-90° by step of 5°. The light source iscombined blue LED and halogen. Data analysis is done by Delta Psi2 software platform.



Microcal VP-ITC MicroCalorimeter :


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The Microcal VP-ITC MicroCalorimeter is a powerful and sensitive, easy-to-use isothermal titration calorimeter that offers the ability to investigate any biomolecular interactions. This fast, convenient, label free and in-solution technique delivers the information needed to characterize the molecular interactions of proteins, antibodies, nucleic acids and other biomolecules. In addition this instrument can assess the driving mechanisms of binding and studying enzyme kinetics and inhibitor design. Isothermal Titration Calorimetry (ITC) measures the binding affinity and thermodynamics between any two biomolecules. When substances bind, heat is either released or absorbed. Measurement of this heat change in an ITC experiment enables accurate determination of binding constants (KD), interaction stoichiometry (n), enthalpy (ΔH) and entropy (ΔS), providing a complete thermodynamic profile of the molecular interaction in a single experiment. This system is equipped with a 300 uL removable buret assembly with the stirring system and a cleaning accessory. The reference and the sample cells have a maximum volume of 1.8 mL with minimum of 1.5 mL is required for the sample cell. In general for DNA and RNA the sample concentration is in the range of 5 μM and 10 μM for protein.


Updated on 12/18/2015