Shared Instrumentation Facility

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The Chemistry Department Shared Instrumentation Facility is housed in Room 357 of the Brown Building and is staffed by Dr. Chin Lin, Dr. Chunhua Hu, and Mr. Ronald McLurkin. The facility houses many of the Department's major research instruments including NMR spectrometers, mass spectrometers, polarimeter, electronic and vibrational spectrophotometers, X-ray diffractometers, and electron microscope.  Also housed here is a phosphorimager and a poster printer.  A diverse community of users access the facility, including faculty, graduate students, undergraduates, and numerous community members.  Colleagues access the facility from other departments, the NYU School of Medicine, and other local institutions.  

The instruments are open to all Chemistry Department research groups.  New users should contact Dr. Chin Lin for training schedules. Researchers outside of the Chemistry Department may also apply to Dr. Lin to use the instruments in the facility.

> Instrument Rate List
> Instrument Reservation System



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The Shared Instrumentation Facility was constructed through the support of the National Center for Research Resources, National Institutes of Health under Research Facilities Improvement Award Number C06 RR-16572-01.



Description of Instruments


Bruker AV-400 High Performance Digital NMR Spectrometer (400 MHz):

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The Bruker Avance-400 NMR spectrometer is equipped with two RF channels, a multinuclear broadband observe (BBO) probe covering the range from 15N to 31P nuclei, an inverse 1H (inner)/ 13C (outer) carbon (SEI) probe, and a GRASP II Gradient Spectroscopy accessory. The BBO probe is further equipped with a built-in Automatic Tuning and Matching Accessory (ATMA) for computer controlled X observation and 1 H decoupling tuning and matching. In addition, the BBO probe was prepared so that the outer coil can be tuned to 19F. A low temperature accessory, including 25 liter nitrogen dewar, nitrogen evaporator, and insulated transfer line, for operation down to -100 °C was also installed. This instrument is accessible to all chemistry research groups except every Thursday morning between 9:00 and 10:30 am (for liquid Nitrogen/Helium filling).

The Bruker Avance-400 NMR Spectrometer was acquired through the support of the National Science Foundation under Award Number CHE-01162222.


Bruker AV-500 High Performance Digital NMR Spectrometer (500 MHz):

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The Bruker Avance-500 NMR spectrometer is equipped with three RF channels, an inverse Triple Resonance Probe (TXI) for observation of 1H while decoupling 13C and 15N nuclei, an inverse (proton-detected) Broadband Probe (BBI) with broadband decoupling coil tunable from 109Ag to 31P nuclei, and a GRASP III three axis Gradient Spectroscopy accessory. The Bruker Avance-500 NMR spectrometer also equipped with a pre-cooling and stabilization accessory (BCU-05) for ultra-precise low temperature control from near ambient conditions down to -5 °C sample temperature ideal for DNA/RNA and peptide molecules.

The Bruker Avance-500 NMR Spectrometer was acquired through the support of the National Science Foundation under Award Number CHE-01162222.


Bruker AVIII-600 High Performance Digital NMR Spectrometer (600 MHz):

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The Bruker AVANCE III-600 NMR spectrometer is equipped with three RF channels, 5 mm sample diameter Inverse Quadruple Resonance Probe (QXI) for observation of 1H while decoupling 13C, 15N, and 31P nuclei, an multinuclear Broadband Fluorine Observe SmartPROBE (BBFO) with digital tuning covering the range from 15N to 31P and 19F nuclei with 1H decoupling, and a GRASP II Z-axis Gradient Spectroscopy accessory. Both QXI and BBFO probes equipped with a computer controlled Automatic Tuning and Matching (ATM) accessory for optimum automation. The Bruker AVANCE III-600 NMR spectrometer also equipped with a pre-cooling and stabilization accessory (BCU-05) for ultra-precise low temperature control from near ambient conditions down to 0 °C sample temperature ideal for biopolymers.

The Bruker AVANCE III-600 NMR Spectrometer was acquired through the support of New York University.


Agilent 1100 Series Capillary LCMSD Trap XCT Spectrometer:

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 The Agilent 1100 Series Capillary LCMSD Trap XCT system is a multi-pole ion-trap mass spectrometer designed to deliver state-of-the-art performance for API ( Atmospheric Pressure Ionization)-LCMS applications for examining polar organic and particularly biological compounds such as DNAs/RNAs and peptides directly in aqueous solution . This system is optimized for capillary LC but still offers a wide range of flow rates. This LCMSD is equipped with a Micro Vacuum Degasser, a Micro Auto-sampler, a Diode Array and Multiple Wavelength Detector, a Thermostatted Column Compartment, and an Ion-trap Mass Selective Detector with a maximum range of 4,000 amu. In addition to ESI ionization source, this system is also equipped with APCI ( Atomic Pressure Chemical Ionization) and APPI ( Atmospheric Pressure Photo Ionization) sources for wide range of samples. In generals, the total amounts of sample injected into the system are in the pmol/μL range with a flow rate from 5 to 200 μL per minute.

The Agilent LCMSD Trap XCT Mass Spectrometer was acquired through the support of the National Science Foundation under Award Number CHE-0234863.


Agilent 6224 Accurate-Mass TOF/LC/MS Spectrometer:

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The Agilent 6224 Accurate-Mass TOF LC/MS system is a High-Definition mass spectrometer capable of delivering exceptional sensitivity and excellent sub 1-ppm mass accuracy. This LC-TOF system is equipped with a vacuum degasser, a binary pump, a well-plate auto-sampler, a diode array and multiple wavelength detector (DAD), and a Time Of Flight (TOF) mass selective detector with a wide mass range of 20,000 m/z. In addition to the duo-ESI ionization source, this system is also equipped with an APCI (Atmospheric Pressure Chemical Ionization) source for wide range of samples with less polarity.

The Agilent 6224 TOF LC/MS Mass Spectrometer was acquired through the support of New York University.


Agilent 1100 Series LCMSD VL MS Spectrometer:

The Agilent 1100 Series LCMSD VL system is a single quadrupole mass spectrometer designed to deliver state-of-the-art performance for API (Atmospheric Pressure Ionization)-LCMS applications. This LCMSD is equipped with a vacuum degasser, a quadruple pump, a well-plate auto-sampler, a diode array and multiple wavelength detector (DAD), and a single quadrupole mass selective detector with a maximum mass range of 1,500 atomic mass unit. In addition to ESI ionization source, this system is also capable of running APCI (Atmospheric Pressure Chemical Ionization) source for wide range of samples with less polarity. In generals, the total concentration of sample injected into the system is in the pmol/µl range with a flow rate up to 1000 µL per minute. 

The Agilent 1100 Series LCMSD VL MS Spectrometer was acquired through the support of New York University.


Bruker UltrafleXtreme MALDI-TOF/TOF:

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The UltrafleXtreme is a MALDI (Matrix Assisted Laser Desorption/Ionization) tandem Mass Spectrometer especially designed for automated MS and MS/MS high throughput identification of proteins and peptides.  Tandem Mass Spectrometry is a technique that utilizes more than one mass selective stage in a mass spectrometer.  The most common form in practice is a two-stage arrangement to record MS/MS or (MS)2 spectra.  Bruker's patented LIFTTM technology allows acquiring full fragment ion spectra within one single scan and replaces traditional measurements of segmented spectra with stepwise-reduced reflector potentials.  The TOF/TOF technology provides enhanced ion optics for cutting-edge MS/MS experiments and second generation PANTM 'panoramic' resolution allows impressive simultaneous mass resolution across a wide mass range leading to reliable top-down sequencing capabilities.

The Bruker Maldi-TOF TOF UltrafleXtreme MS Spectrometer was acquired through the support of the National Science Foundation under Award Number CHE-0958457.


ThermoFinnigan PolarisQ ion-trap GCMS Spectrometer:

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The departmental PolarisQ GCMS spectrometer is equipped with an ion-trap Mass Selective Detector capable of detecting small volatile organic molecules from 10 up to 1,000 Dalton with unit mass resolution across the entire mass range. The Trace Gas Chromatography is equipped with single channel digital pressure/flow control capable of multilevel temperature control with a maximum oven temperature of 450 °C. This GCMS spectrometer is capable of performing EI, CI positive, and CI negative ionization modes.  Typical injection amount is in the range of 10 pg/μl.

The ThermoElectron GCMS Spectrometer was acquired through the support of the National Science Foundation under Award Number DUE-0126958.


Thermo Nicolet AVATAR FTIR Spectrometer:

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The Chemistry Department FT-IR spectrometer is a Thermo Nicolet AVATAR 360 Fourier Transform Infrared Spectrometer capable of discerning what functional groups are present in a molecule. Infrared spectroscopy (IR) is based on the interaction of the vibration frequencies of individual and groups of bonds within a molecule with the infrared portion of the electromagnetic spectrum.

The Thermo Nicolet AVATAR FTIR Spectrometer was acquired through the support of New York University.


Cary 100 Bio UV-Visible Spectrophometer:

 The Cary 100 Bio spectrophotometer is a mid-priced instrument, ideal for routine laboratory work and is controlled by the Cary WinUV software. This Windows based software features a modular design which makes it easy to use. It has a working range past 3.5 absorbance units (AU) and variable slits. The instrument comes with a 6 x 6 peltier multicell holder and a temperature controller unit. The peltier thermostattable multicell holder features, use of rotating magnetic fields to provide magnetic stirring in each of the 12 cells, uilt-in cell lifting, Peltier blocks fitted down both sides of the cuvette positions, and a water channel that evenly encircles the cell block. The temperature range is from about -10 deg C to 100 deg C, ideal for DNA/RNA melting temperature determination.

The Cary 100 Bio UV-Visible Spectrophometer was acquired through the support of New York University.


Storm PhosphorImager:

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The Molecular PhosphorImager systems Storm 840 provides users PhosphorImager capability for autoradiography, direct fluorescence for nucleic acid and protein gel analysis, and chemifluorescence for fast blot analysis without exposures. Please clean up the scanner screen with mixture of ethanol/water after each use.

The Storm PhosphorImager was acquired through the support of New York University.


Circular Dichroism Spectropolarimeter:

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The Department has an Aviv Stopped Flow CD Spectropolarimeter Model 202SF equipped with a thermostated five compartments cell holder. Circular Dichroism is an excellent tool for analyzing peptide/protein and nucleic acid (DNA and RNA) secondary structure in solution. It can be used to follow the changes in folding as a function of temperature, and is also useful for measuring protein-ligand and nucleic acid-ligand interactions.

The Circular Dichroism Spectropolarimeter was acquired through the support of New York University.


Perkin Elmer 2400 Series II CHNS/O Elemental Analyzer:

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The system can determine weight percent carbon, hydrogen, nitrogen, sulfur or oxygen in a variety of sample types, and operate in 3 modes: CHN, CHNS and Oxygen. Currently the system is only operating under the CHN mode. A typical measurement requires 1.5-2.5 mg of sample. Please contact Dr. Chunhua "Tony" Hu for training.


Bruker AXS SMART APEXII Single Crystal Diffractometer:

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It is equipped with an APEXII CCD area detector and an Oxford Cryosystems 700 plus Cooler. It is not only used for single crystal structure determination, also can be applied to powder sample for phase identification. Please visit the X-ray Diffraction Facility web page for more information.

The Bruker AXS SMART APEXII Single Crystal Diffractometer was acquired through the support of the Molecular Design Institute in the Department of Chemistry at New York University.


Bruker AXS D8 DISCOVER with GADDS Diffractometer:

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Please visit the X-ray Diffraction Facility web page for more information.

The X-ray microdiffractometer with GADDS was acquired through the support of the National Science Foundation under Award Numbers CRIF/CHE-0840277 and Award Number MRSEC/DMR-0820341.


Merlin FESEM:

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MERLIN (Carl Zeiss) field emission scanning electron microscope (FESEM): The Merlin FESEM features standard detectors, ‘SE2’ (Everhart-Thornley type) and ‘in lens’ (annular secondary electron).

The Merlin FESEM was acquired through the support of the MRI program of the National Science Foundation under Award Number DMR-0923251.

Please visit the Electron Microscopy Facility web page for more information.

Updated on 08/04/2012