The Chemistry Department Instrumentation Center is housed in Room 353 of the Brown Building and is managed by
Dr. Chin Lin and
Mr. Ronald McLurkin. The facility houses many of the Department's major research instruments including NMR spectrometers, mass spectrometers, polarimeter, and electronic and vibrational spectrophotometers. Also housed here is a phosphorimager and a poster printer. A diverse community of users access the facility, including faculty, graduate students, undergraduates, and numerous community members. Colleagues access the facility from other departments, the NYU School of Medicine, and other local institutions.
The instruments are open to all Chemistry Department research groups. New users should contact Dr. Chin Lin for training schedules. Researchers outside of the Chemistry Department may also apply to Dr. Lin to use the instruments in the facility.
The facility was constructed with support from Research Facilities Improvement Grant Number C06 RR-16572-01 from the National Center for Research Resources, National Institutes of Health. Several of the instruments were purchased with financial support from the National Science Foundation (Grants CHE-0234863, CHE-0116222, and DUE-0126958).
Descriptions of Instruments
Bruker AV-400 High Performance Digital NMR Spectrometer (400 MHz): The Bruker Avance-400 NMR spectrometer is equipped with two RF channels, a multinuclear broadband observe (BBO) probe covering the range from 15N to 31P nuclei, an inverse 1H (inner)/ 13C (outer) carbon (SEI) probe, and a GRASP II Gradient Spectroscopy accessory. The BBO probe is further equipped with a built-in Automatic Tuning and Matching Accessory (ATMA) for computer controlled X observation and 1 H decoupling tuning and matching. In addition, the BBO probe was prepared so that the outer coil can be tuned to 19F. A low temperature accessory, including 25 liter nitrogen dewar, nitrogen evaporator, and insulated transfer line, for operation down to -100 °C was also installed. This instrument is accessible to all chemistry research groups except every Thursday morning between 9:00 and 10:30 am (for liquid Nitrogen/Helium filling). To sign up for usage please go to http://chemistry.fas.nyu.edu/object/chem.resources.deptservices. This instrument was purchased with financial support from NSF grant CHE-01162222.
Bruker AV-500 High Performance Digital NMR Spectrometer (500 MHz):The Bruker Avance-500 NMR spectrometer is equipped with three RF channels, an inverse Triple Resonance Probe (TXI) for observation of 1H while decoupling 13C and 15N nuclei, an inverse (proton-detected) Broadband Probe (BBI) with broadband decoupling coil tunable from 109Ag to 31P nuclei, and a GRASP III three axis Gradient Spectroscopy accessory. The Bruker Avance-500 NMR spectrometer also equipped with a pre-cooling and stabilization accessory (BCU-05) for ultra-precise low temperature control from near ambient conditions down to -5 °C sample temperature ideal for DNA/RNA and peptide molecules.To sign up for usage please go to http://chemistry.fas.nyu.edu/object/chem.resources.deptservices. This instrument was purchased with financial support from NSF grant CHE-0116222.
Agilent 1100 Series Capillary LCMSD Trap XCT Spectrometer:The Agilent 1100 Series Capillary LCMSD Trap XCT system is a multi-pole ion-trap mass spectrometer designed to deliver state-of-the-art performance for API ( Atmospheric Pressure Ionization)-LCMS applications for examining polar organic and particularly biological compounds such as DNAs/RNAs and peptides directly in aqueous solution . This system is optimized for capillary LC but still offers a wide range of flow rates. This LCMSD is equipped with a Micro Vacuum Degasser, a Micro Auto-sampler, a Diode Array and Multiple Wavelength Detector, a Thermostatted Column Compartment, and an Ion-trap Mass Selective Detector with a maximum range of 4,000 amu. In addition to ESI ionization source, this system is also equipped with APCI ( Atomic Pressure Chemical Ionization) and APPI ( Atmospheric Pressure Photo Ionization) sources for wide range of samples. In generals, the total amounts of sample injected into the system are in the pmol/μL range with a flow rate from 5 to 200 μL per minute.To sign up for usage please go to http://chemistry.fas.nyu.edu/object/chem.resources.deptservices. This instrument was puchased with financial support from NSF grant CHE-0234863.
Bruker OmniFLEX MALDI-TOF MS Spectrometer:The Bruker OmniFLEX MALDI-TOF Mass Spectrometer provides one unique way to ionize nonvolatile (organic) samples with low to high molecular weight up to 132,000 Dalton. MALDI is extraordinary sensitive such that the total amounts of sample loaded onto the target surface are in the picomole (10-12) to femtomole (10-15) range. The OmniFLEX MALDI contains a pulsed ultraviolet nitrogen laser, 200 μJ at 337 nm and a Time-of-Flight mass analyzer with a 120 cm linear flight path. Typical matrices used for MALDI analysis are α-cyano-4-hydroxycinnamic acid for peptides, 3-hydroxypicolinic acid for oligonucleotides, and 2,5 -dihydroxybenzoic acid for carbohydrates, aromatics, and small organic molecules.To sign up for usage please go to http://chemistry.fas.nyu.edu/object/chem.resources.deptservices.
ThermoFinnigan PolarisQ ion-trap GCMS Spectrometer:The departmental PolarisQ GCMS spectrometer is equipped with an ion-trap Mass Selective Detector capable of detecting small volatile organic molecules from 10 up to 1,000 Dalton with unit mass resolution across the entire mass range. The Trace Gas Chromatography is equipped with single channel digital pressure/flow control capable of multilevel temperature control with a maximum oven temperature of 450 °C. This GCMS spectrometer is capable of performing EI, CI positive, and CI negative ionization modes.To sign up for usage please go to http://chemistry.fas.nyu.edu/object/chem.resources.deptservices. Typical injection amount is in the range of 10 pg/μl. This instrument was purchased with financial support from NSF grant DUE-0126958.
ThermoNicolet AVATAR FTIR Spectrometer:The chemistry department FT-IR spectrometer is a ThermoNicolet AVATAR 360 Fourier Transform Infrared Spectrometer capable of discerning what functional groups are present in a molecule. Infrared spectroscopy (IR) is based on the interaction of the vibration frequencies of individual and groups of bonds within a molecule with the infrared portion of the electromagnetic spectrum.
Storm PhosphorImager:The Molecular PhosphorImager systems Storm 840 provides users PhosphorImager capability for autoradiography, direct fluorescence for nucleic acid and protein gel analysis, and chemifluorescence for fast blot analysis without exposures. Please clean up the scanner screen with mixture of ethanol/water after each use.
Circular Dichroism Spectropolarimeter:The Department has an Aviv Stopped Flow CD Spectropolarimeter Model 202SF equipped with a thermostatted five compartments cell holder. Circular Dichroism is an excellent tool for analyzing peptide/protein and nucleic acid (DNA and RNA) secondary structure in solution. It can be used to follow the changes in folding as a function of temperature, and is also useful for measuring protein-ligand and nucleic acid-ligand interactions.
Instrument Rate List
